chicken anti lacz  (AvesLabs)

Journal: bioRxiv

Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling

doi: 10.1101/2024.04.03.587940

Figure Lengend Snippet: (A-C) Wing discs of hs-Flp ; ds Cr+:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (A) , hs-Flp ; d sCr Δ A:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (B), or hs-Flp ; ds Cr Δ D:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (C) , with mitotic clones homozygous for ds Cr+:V5 (A), d sCr Δ A:V5 (B), or ds Cr Δ D:V5 (C), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (D-F) Wing discs of hs-Flp ; ds Cr+:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (D) , hs-Flp ; ds Cr Δ A:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (E) , or hs-Flp ; ds Cr Δ D:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (F) stained for Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (G) Histogram showing classification of Dachs polarity in Flp-out clones in different ds genotypes. 60 clones were scored per genotype. (H) Western blot showing results of co-immunoprecipitation experiments between V5-tagged Ds proteins and FLAG-tagged Dachs transfected into S2 cells. Top three panels show blots on total cell lysates transfected to express the indicated proteins, using the antibodies indicated at right. Bottom two panels show blots on proteins immunoprecipitated with anti-V5 beads and detected with V5 or FLAG antibodies.

Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).

Techniques: Clone Assay, Staining, Expressing, Western Blot, Immunoprecipitation, Transfection

Journal: bioRxiv

Article Title: Contributions of the Dachsous intracellular domain to Dachsous-Fat signaling

doi: 10.1101/2024.04.03.587940

Figure Lengend Snippet: (A-F) Wing discs from homozygous ds CrD Δ II:V5 (A) or ds CrD Δ III:V5 (D) stained for Ds:V5. Red square boxes in (A) show approximate locations in the hinge and wing pouch (WP) corresponding to the higher magnification panels at right. ( A,D ) Lower magnification images showing the wing pouch and hinge regions, Scale bar = 100 µm. (B,C,E,F) Higher magnification images depicting part of the wing hinge ( B,E ) or proximal wing pouch ( C,F ) in discs expressing V5-tagged Ds from ds CrD Δ II:V5 ( B,C ) or ds CrD Δ III:V5 ( E,F ). Scale bar = 10 µm. (G,H) Wing discs from hs-Flp ; ds CrD Δ II:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (G) , or hs-Flp ; ds CrD Δ III:V5 FRT40A/arm-lacZ FRT40A; Dachs:GFP/+ (H), with mitotic clones homozygous for ds CrD Δ II:V5 (G), or ds CrD Δ III:V5 (H), marked by loss of ß-gal (red), outlined with white dotted lines, to show effects on Dachs:GFP (green). (I,J) Wing discs from hs-Flp ; ds CrD Δ II:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (I) , or hs-Flp ; ds CrD Δ III:V5 /ds ΔEx12 ; Act>stop>EGFP:Dachs/+ (J) , stained for expression of Wg (white) and E-cad (red) and with Flip-out clones expressing Dachs:GFP (green/white). Panels at right show Dachs:GFP (white) in higher magnification images of the boxed regions. Proximal (P) to distal (D) orientation is indicated. (K) Histogram showing the classification of Dachs polarity in Flp-out clones in different ds genotypes as normal (blue color) or mispolarised (orange). N= number scored.

Article Snippet: Primary antibodies were used for staining were: Mouse anti-V5 (Invitrogen R960-25; preabsorbed at 1:10 dilution, then used at 1:50), Rat anti-E-cadherin (DSHB DCAD2-c 1:200), Chicken anti-ß-gal (Abcam ab9361; 1:100), Rat anti-Fat (1:1500) , Mouse anti-Wg (DSHB 9A4; 1:300), Rabbit anti-Dcr2 (Abcam ab4732, 1:1600), Rabbit anti-FLAG (Sigma F3165; 1:200).

Techniques: Staining, Expressing, Clone Assay

Journal: eLife

Article Title: Retinoic acid signaling mediates peripheral cone photoreceptor survival in a mouse model of retina degeneration

doi: 10.7554/eLife.76389

Figure Lengend Snippet:

Article Snippet: Antibody , (Chicken polyclonal) anti-B-galactosidase , Aves Labs , BGL1010 , (1:3000).

Techniques: Recombinant, Expressing, Plasmid Preparation, Sequencing, RNAscope